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IDH1 (R132H) mouse monoclonal antibody 点突变抗体

产品信息
  • IDH1 (R132H) [26081]
    [30 µL]    		  

     

    Catalog Number:  26081
    Gene Symbol:  IDH1
    Description:   Anti-IDH1 (R132H) Mouse Monoclonal Antibody
    Background:   Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-132 in IDH to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.
    Immunogen:   Recombinant protein of human IDH1 which includes the mutation of R132H.
    Applications:   ELISA, WB, IHC, IP
    Recommended Dilutions:    
    ELISA: 1:1000-1:5000 
    WB: 1:100-1:1000 
    IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti-IDH1(R132H) antibody recognizes IDH1(R132H) of vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    Western blot:  
    Western blot analysis of recombinant IDH1(R132H) and wild-type proteins. Purified His-tagged IDH1 (R132H) protein (Cat.#10199) and wild-type protein (amino acid 1-240) (Cat.#10198) were blotted with anti-IDH1(R132H) mouse antibody (Cat.# 26081)(Left panel) or with anti-IDH1 mouse antibody (Cat. #26136 right panel).
    Immunofluorescence:  
    Immunofluorescence of cells expressing IDH1 proteins with anti-IDH1(R132H) antibody. HEK293T cells were transfected with pCDNA3-GFP-IDH1 (WT) plasmid (left column) or pCDNA3-GFP-IDH1 (R132H) plasmid (right column), then fixed and stained with anti-IDH1 (R132H) monoclonal antibody (Cat. #26081).
     
    Related Products
    Catalog# Name Size
    26136 Anti-IDH1 Mouse Monoclonal Antibody  100 μL
    21277 Anti-IDH1 Rabbit Polyclonal Antibody  100 μL
    26081 Anti-IDH1 (R132H) Mouse Monoclonal Antibody  100 μL
    26160 Anti-IDH1 (R132S) Mouse Monoclonal Antibody  100 μL
    21278 Anti-IDH2 Rabbit Polyclonal Antibody  100 μL
    26084 Anti-IDH2 (R172H) Mouse Monoclonal Antibody  100 μL
    26163 Anti-IDH2 (R172K) Mouse Monoclonal Antibody  100 μL
    26164 Anti-IDH2 (R172W) Mouse Monoclonal Antibody  100 μL
    26321 Anti-IDH2 (R172G) Mouse Monoclonal Antibody  100 μL
    26165 Anti-IDH2 (R140Q) Mouse Monoclonal Antibody  100 μL
    26406 Anti-IDH1 (R132P) Mouse Monoclonal Antibody  100 μL
    26408 Anti-IDH2 (R172S) Mouse Monoclonal Antibody  100 μL
    Publications:
    1.  Specific monoclonal antibodies against IDH1/2 mutations as diagnostic tools for gliomas
        Brain Tumor Pathology. January 2015, Volume 32, Issue 1, pp 3-11
    2.  Mutant IDH inhibits HNF-4α to block hepatocyte differentiation and promote biliary cancer
        Nature 513, 110–114 (04 September 2014)
    3.  Metabolomic comparison between cells overexpressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism
        Journal of Neurochemistry. Volume 132, Issue 2, pages 183–193, January 2015

     
     

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