Product Description: Affinity purified rabbit polyclonal anti-phosphothreonine antibody (anti-pT), was developed using phosphothreonine-KLH conjugates and phosvitin mixture as immunogen. The phosphothreonine specific antibody was affinity purified with phoshothreonine immobilized on agarose. Species: Rabbit Formulation: 250 μg/mL antibody stored in PBS, pH 7, with 50% glycerol. Storage & Stability: Store product at –20℃. One year from date of shipping. Immunogen: Phosphothreonine-KLH conjugates and Phosvitin. Purification: The antibody was affinity purified with phosphothreonine on agarose followed by affinity adsorption with threonine on agarose. Specificity: Recognize peptide and proteins phosphorylated on threonine residues. No cross-reaction with phosphotyrosine. Tested: Human Mouse Application(s): ELISA, WB, IP,IHC. Scientific Description: Threonine is one of the 20 naturally occurring amino acids. Kinases phosphorylate threonine to phosphothreonine which enters various biosynthetic pathways in the body. References: M.J. Betts, R.B. Russell. Bioinformatics for Geneticists, M.R. Barnes, I.C. Gray eds, Wiley, 2003.
