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DIG-High Prime DNA Labeling and Detection Starter Kit II Roche 11585614910

产品信息
  •                 DIG-High Prime DNA Labeling and Detection Starter Kit II
                           Kit for chemiluminescent detection with CSPD, ready-to use.

    产品应用:
    DIG-High Prime labeled probes can be used in northern, Southern-, and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.
    产品介绍:
    Sensitivity and specificity:Standard assay uses 1 µg of linearized pBR328. It is usual to expect 0.8 µg labeled DNA after 1 h of labeling, and 2.3 µg labeled DNA after 20 h reaction. In a dot blot hybridization, 0.03 pg of homologous DNA is detectable with chemiluminescence (probe concentrations at 20 ng/ml). A single copy human gene (t-PA) is detectable in a Southern blot, loading 1 µg of digested placenta DNA.
    组分:
    1. DIG-High Prime, 5x conc., 50 µl
    2. DIG-labeled Control DNA, 20 µl, (5 µg/ml) pBR328 (linearized with Bam HI)
    3. DNA Dilution Buffer, 3 x 1 ml
    4. Anti-digoxigenin-AP Conjugate, 50 µl
    5. CSPD, ready-to-use, 50 ml
    6. Blocking Solution, 10x conc., 4 x 100 ml
    7. DIG Easy Hyb Granules, 4 x 100 ml
    产品信息
    产地 产品名称 产品货号 规格 目录价
    Roche DIG-High Prime DNA Labeling and Detection Starter Kit II 11585614910 kit 4764.24
    促销价:4336.00

     
    上海前尘生物科技有限公司,是一家专注于生命科学和生物技术领域的高新科技企业。是国内代理,销售,研发为一体的生物技术公司。为全国各科研单位提供优质的产品与服务。
    温馨提示:不可用于临床ZL。
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