Recombinant Human FGF-basic,重组人FGF-basic

 Recombinant Human FGF-basic,重组人FGF-basic

品名:Recombinant Human FGF-basic
产地：PeproTech
货号：100-18B
Cross Reactivity: Cow, Dog, Frog, Hamster, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Human + Mouse, Rat + Chicken
Biological Activity: Assay #1: The as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 106 units/mg.
Assay #2: The was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
Description: FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells ａnd endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c ａnd 4. Recombinant human FGF-basic is a 17.2 kDa protein consisting of 154 amino acid residues. FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development ａnd postnatal growth ａnd regeneration of a variety of tissues, by promoting cellular proliferation ａnd differentiation.

 Recombinant Human FGF-basic,重组人FGF-basic 操作步骤
1.标准品的稀释：本试剂盒提供原倍标准品一支，用户可按照下列图表在小试管中进行稀释。

2.加样：分别设空白孔（空白对照孔不加样品及酶标试剂，其余各步操作相同）、标准孔、待测样品孔。在酶标包被板上标准品准确加样50μl，待测样品孔中先加样品稀释液40μl，然后再加待测样品10μl（样品稀释度为5倍）。加样将样品加于酶标板孔底部，尽量不触及孔壁，轻轻晃动混匀。
3.温育：用封板膜封板后置37℃温育30分钟。
4.配液：将30倍浓缩洗涤液用蒸馏水30倍稀释后备用。
5.洗涤：小心揭掉封板膜，弃去液体，甩干，每孔加满洗涤液，静置30秒后弃去，如此重复5次，拍干。
6.加酶：每孔加入酶标试剂50μl，空白孔除外。
7.温育：操作同3。
8.洗涤：操作同5。
9.显色：每孔先加入显色剂A50μl，再加入显色剂B50μl，轻轻震荡混匀，37℃避光显色15分钟。
10. 终止：每孔加终止液50μl，终止反应（此时蓝色立转黄色）。
11. 测定：以空白空调零，450nm波长依序测量各孔的吸光度（OD值）。 测定应在加终止液后15分钟以内进行。