外泌体exosomes纯化试剂，4478359 Total Exosome Isolation

Total Exosome Isolation Reagent (from cell culture media) enables fast ａnd efficient enrichment of intact exosomes from cell culture media samples.
• Maximize recovery of intact exosomes for any type of downstream application
• Easily isolate exosomes using our simple ａnd reliable protocol
• Avoid time-consuming ultra-centrifugation
• Maximize flexibility—can be scaled up or down depending on the sample size

Isolation of exosomes can be a tedious, non-specific, ａnd difficult process. Total Exosome Isolation Reagent (from cell culture media) enables simple ａnd reliable concentration of intact exosomes from cell media samples with a protocol that is scalable depending on your sample size.

By tying up water molecules, the reagent forces less-soluble components such as vesicles out of solution, allowing them to be collected by a short, low-speed centrifugation. The reagent is added to the cell media sample, ａnd the solution is incubated overnight at 2°C to 8°C. The precipitated exosomes are recovered by standard centrifugation at 10,000 x g for 60 min. The pellet is then resuspended in PBS or similar buffer, ａnd the exosomes are ready for downstream analysis or further purification by affinity methods. Total RNA ａnd protein can then be purified using the Total Exosome RNA ａnd Protein Isolation Kit, or the intact exosomes can be used for biological studies of their pathways, functions, ａnd trafficking. For immunoprecipitation of exosomal proteins, use Exosome Immunoprecipitation (Protein A) or Exosome Immunoprecipitation (Protein G).

Store at 2 to 8 °C.