产品编号Rs-11810R 英文名称BRSK2 中文名称脑蛋白丝氨酸/苏氨酸激酶29抗体 别 名BR serine/threonine kinase 2; BR serine/threonine-protein kinase 2; Brain selective kinase 2; Brain specific serine/threonine protein kinase 2; Brsk2; BRSK2_HUMAN; C11orf7; PEN11B; Protein kinase SAD1B; SAD1; Serine/threonine kinase 29; Serine/threonine protein kinase BRSK2; Serine/threonine-protein kinase 29; Serine/threonine-protein kinase SAD-A; STK29; SADA. 说 明 书0.1ml 0.2ml 研究领域免疫学 神经生物学 激酶和磷酸酶 表观遗传学 抗体来源Rabbit 克隆类型Polyclonal 交叉反应Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep, 产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. 分 子 量82kDa 性 状Lyophilized or Liquid 浓 度1mg/1ml 免 疫 原KLH conjugated synthetic peptide derived from human STK29 亚 型IgG 纯化方法affinity purified by Protein A 储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 ａnd 50% Glycerol. 保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. PubMedPubMed 产品介绍background: The phosphorylation ａnd dephosphorylation of proteins on serine ａnd threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis ａnd apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases. BRSK2 (BR serine/threonine kinase 2), also known as SAD1, STK29 or PEN11B, is a 736 amino acid protein that contains one protein kinase domain ａnd is preferentially expressed in brain ａnd testis. One of several members of the Ser/Thr protein kinase family, BRSK2 uses magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins ａnd is thought to be involved in microtubule assembly, neuronal polarization ａnd synaptic development. Additionally, BRSK2 may function as an autoantigen involved in small-cell lung cancer-associated limbic encephalitis. Five isoforms of BRSK2 exist due to alternative splicing events.
Function: Required for the polarization of forebrain neurons which endows axons ａnd dendrites with distinct properties, possibly by locally regulating phosphorylation of microtubule-associated proteins.
Subcellular Location: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. AMPK subfamily. Contains 1 protein kinase domain.
Post-translational modifications: Phosphorylated at Thr-174 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase ａnd CAB39. Not phosphorylated at Thr-174 by CaMKK2. In contrast, it is phosphorylated ａnd activated by CaMKK1. May be inactivated via dephosphorylation of Thr-174 by PP2C. May be phosphorylated at Thr-260 by PKA. However, phosphorylation at Thr-260 by PKA was not comfirmed later (PubMed:18339622).
Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. Contains 1 protein kinase domain. Contains 1 UBA domain.
Database links: Entrez Gene: 9024 Human Entrez Gene: 75770 Mouse Omim: 609236 Human SwissProt: Q8IWQ3 Human SwissProt: Q69Z98 Mouse Unigene: 170819 Human Unigene: 274868 Mouse Unigene: 482784 Mouse
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
