产品编号Rs-4291R
英文名称BARD1
中文名称乳腺癌易感基因环状结构域蛋白抗体
别 名BARD-1; Bard1; Bard 1; BARD1_HUMAN; BRCA1 associated RING domain 1; BRCA1 associated RING domain gene 1; BRCA1 associated RING domain protein 1; BRCA1-associated RING domain protein 1.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 免疫学 染色质和核信号 细胞凋亡
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse,
产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量85kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human BARD1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 ａnd 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
BRCA1 Associated RING Domain gene 1 (BARD1) interacts with the N terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo ａnd in vitro, BARD1 shares homology with the 2 most conserved regions of BRCA1: the N terminal RING motif ａnd the C terminal BRCT domain. The RING motif is a cysteine rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes ａnd dominant protooncogenes. The BARD1 protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. BARD1 may be the target of oncogenic mutations in breast or ovarian cancer. BARD1 also plays a role in mediating apoptotic stress ａnd p53 dependent.
Subunit:
Homo- ａnd heterodimer. Heterodimer
Subcellular Location:
Nucleus.
Similarity:
Contains 4 ANK repeats.
Contains 2 BRCT domains.
Contains 1 RING-type zinc finger.
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
