技术文章库
Rat Cyclin-D2 ELISA Kit使用原理
大鼠细胞周期素D2(Cyclin-D2)ELISA试剂盒
货号:FK-QZ1997
规格：96T/48T
Rat Cyclin-D2 ELISA Kit使用原理
The kit was a solid phase sandwichenzyme-linked immunosorbent assay (ELISA), and the AVP concentrations ofthe standard samples and unknown concentrations of samples were detectedby the addition of microporous enzyme plates.. At the same time, the AVPand the biotin labeled antibodies were incubated at the same time..After washing, the addition of Pro labeled HRP. After the warming andwashing, the removal of the non binding enzyme conjugate, and then addedto the substrate a, B, and the enzyme conjugate at the same time.Produce color. The color depth and the concentration of AVP in thesample was proportional to the concentration of the sample..
Operation notes
2 the experimental plate should be immediately put back in the bag,seal the preservation, lest metamorphism.
4 use a disposable suction head lest cross contamination, draw thetermination liquid and substrate B, a liquid, to avoid using the metalpart of the sample.
6 wash the enzyme plate should be fully dry, do not absorb water paperdirectly into the enzyme labeled pores in the water absorption.
8 add reagents should be consistent in order, to ensure that allreaction plate hole incubation time.
1 the correlation coefficient r value of linear regression and theexpected concentration of sample was 0.92 above.
Detection range:
Save condition and its validity:
2 validity: 6 months
