技术文章库

使用复合模式SPE技术去除血浆样品中的聚乙二醇400(三)

将PEG 400从血浆中去除的主要目的就是为了清除可能引发的离子YZ效应。图5为由下面方程式得出的基质效应数据一览,据ZXAAPS论文报道6。

基质效应 =(含PEG的峰响应值/不含PEG的峰响应值)

结论

1. Tong X, Wang J, Zheng S, Pivnichny J, Griffin P, Shen X, Donnelly M, Vakerich K, Nunes C, and Fenyk-Melody J. Effect of signal interference from dosing excipients on pharmacokinetic screening of drug candidates by liquid chromatography/mass spectrometry. Anal Chem 2002 74:6305-6313.
3. Shou W and Naidong W. Post-column infusion stude of the ‘dosing vehicle effect’ in the liquid chromatography/tandem mass spectrometric analysis of discovery pharmacokinetic samples. Rapid Comm. Mass Spec 2003 17:589-597.
5. Larger P, Breda M, Fraier D, Hughes H, and James C. Ion-suppression effects in liquid chromatography-tandem mass spectrometry due to a formulation agent, a case study in drug discovery bioanalysis. J. Pharm. Biomed. Anal. 2005 39:206-216.


2016-06-16
您可能感兴趣的技术文章