GE 表面活性剂 Silwet L-77 说明书

 GE  表面活性剂  Silwet L-77 说明书

• 表面活性剂Silwet L-77是拟喃芥、油菜、大肠杆菌等转化必须试剂之一，原产地为美国GE公司，GE原厂货号为SL77080596-10ml，SL77080596-35ml，我公司再提供10ML规格的分装产品。

   

  Silwet L-77说明书

   

  注意：近期市场上出现假冒SILWET L-77一般为NP40等表面活性物质，外观和SILWET L-77相似，但转化效率非常低，的转化效率也只有SILWET L-77的1/40，假冒SILWET L-77一般只能提供10ML分装产品，我公司有10ML原装和35ML原装，并可提供相关进口证明材料，我公司也可提供相关转化实验服务，费用请电话咨询。

  Silwet L-77 is a chemical mixture of polyalkyleneoxide modified heptamethyltrisiloxane （84%） ａnd allyloxypolyethyleneglycol methyl ether （16%）. It is an essential infiltration media ingredient for all Agrobacterium-based transformations of Arabidopsis ａnd other plants.10 mL ａnd 35 mL are sufficient quantity to prepare 33 L ａnd 115 L of vacuum infiltration medium when used at the recommended rate of 0.03%, v/v, respectively.

  SILWET L-77 is a unique surfactant designed to improve the spreading ａnd wetting properties of pesticide spray mixes. SILWET L-77 utilizes unique organosilicone chemistry which provides the most complete coverage possible. SILWET L-77 can improve cuticular penetration of plant surfaces by the spray mix. Optimum application ａnd resultant effects can be influenced by many factors. Consequently, it is recommended that careful observation of the spray deposit be made ａnd adjuvant rates adjusted accordingly. Applications should be made to ensure thorough coverage without excessive run-off.

  TYPICAL PROPERTIES
  Physical appearance Clear, light amber liquid
  Pounds per gallon 8.370
  Specific gravity 1.0 - 1.01
  pH （1.0%） 6.0 - 7.0
  Freeze Point less than 35 degrees F
  Surface Tension （0.125% v/v） less than 25 dynes/cm
  Contact Angle （0.125% v/v） less than 5 degrees F
  Draves Wet Time （0.125% v/v） less than 15.0 seconds

  GENERAL PRODUCT INFORMATION
  （1） SILWET L-77 contains organosilicone surfactants designed to provide superior wetting ａnd coverage.
  （2） SILWET L-77 can improve water penetration of hard to wet soils ａnd provide more uniform distribution of applied moisture.
  （3） SILWET L-77 is generally compatible with pesticides ａnd fertilizer solutions.
  （4） SILWET L-77 may cause excessive foam if not properly added to the spray tank. To minimize foam generation, add SILWET L-77 to the tank after all other ingredients are added. If foaming is still a concern, add an antifoaming agent, such as FOAMBUSTER or COMBAT PLUS to the tank prior to adding SILWET L-77.
  （5） Do not allow spray mixes containing SILWET L-77 to sit over 36 hours.
  

  拟南芥的种植（拟南芥种植，拟南芥转化，油菜转化）

  拌土：蛭石:黑土:珍珠岩=3:1:0.5用拟南芥PNS营养液浇，然后再将拟南芥种子点到土上。再用保鲜膜封住，再用报纸盖上数日后取走报纸，每天光照16小时。

  拟南芥PNS营养液

                1M磷酸缓冲液（PH5.5）       2.5ml

                1M KNO₃                                   5ml

                1M MgSO₄·7H₂O                  2ml 

                1M Ca（NO₃）₂·4 H₂O             2ml

                20mM Fe·EDTA                   2.5ml

                MS微量元素                        1ml

                加水定容至1L

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