DIGT-250 谷胱甘肽测试盒 | QuantiChrom™ Glutat...

产品信息

谷胱甘肽测试盒 | QuantiChrom™ Glutathione Assay Kit

产品说明  

谷胱甘肽是由甘氨酸、谷氨酸和半胱氨酸构成的三肽。在血红细胞里，还原型谷胱甘肽对于维持还原态血红素和保护细胞免于氧化非常重要。谷胱甘肽参与谷胱甘肽氧化酶去除过氧化氢毒性的过程。在谷胱甘肽的代谢中,缺乏葡萄糖磷酸脱氢酶、谷胱甘肽合成酶或谷胱甘肽还原酶等主要酶的作用，会造成谷胱甘肽的含量降低。在科研和药物研发中，方便、直接、自动化的还原型谷胱甘肽浓度检测手段是Z为理想的。本公司研制的谷胱甘肽检测试剂盒用于从生物样本中准确的检测还原型谷胱甘肽, 反应产生黄色产物特性，在波长为412nm下检测，其吸光度与样本中谷胱甘肽的含量对成正比。

测定方法  比色法（412nm）定量测定还原型谷胱甘肽的浓度。

产品特点  

敏感、准确：在96孔板中测试，谷胱甘肽的线性测试范围在0.4-100μM。

操作简便：整个测试过程是将样本与DTNB试剂混合，去除蛋白质沉淀，然后加入第二种试剂并检测吸光度。

产品用途  

1．直接检测全血、血清、血浆、尿液、组织和细胞提取物中的还原型谷胱甘肽含量。

2．适用于新药开发和药理学研究，检测药物对谷胱甘肽代谢的影响。

储藏条件  可在室温下运输。试剂在4℃环境下保存。

DESCRIPTION  Glutathione is a tripeptide of glycine, glutamic acid and cysteine. In the red blood cell, the reduced form of glutathione is vital in maintaining hemoglobin in a reduced state and hence protecting the cells from oxidative damage. Glutathione is involved in detoxification of hydrogen peroxide through glutathione oxidase. Low levels of glutathione are found in deficiencies of key enzymes involved in glutathione metabolism, such as glucose-6-phosphate dehydrogenase, glutathione synthase and glutathione reductase. Simple, direct and automation-ready procedures for measuring reduced glutathione are becoming popular in Research and Drug Discovery. Our Assay Kit is designed to accuray measure reduced glutathione in biological samples. The optical density of the formed yellow product, measured at 412 nm, is directly proportional to glutathione concentration in the sample.

KEY FEATURES  Sensitive and accurate. Linear detection range 0.4 - 100 μM in 96-well plate. Simple and convenient. The procedure involves mixing the Reagent with sample, removing protein precipitates for proteinaceous samples, adding a second Reagent and reading the optical density. Low interference. Amino acids and common buffers do not interfere.

APPLICATIONS  Direct Assays: reduced glutathione in whole blood, plasma, serum, urine, tissue and cell extracts. Drug Discovery/Pharmacology: effects of drugs on glutathione metabolism.

STORAGE CONDITIONS  The kit is shipped at room temperature. Store all components at 4°C. Shelf life: 6 months.