产品名称：Agarose Gel DNA Extraction Kit
产品货号：11696505001
产品规格：1 kit （for max. 100 reactions）
产品价格：1597
产品应用：The kit can efficiently isolate both small and large DNA fragments from standard or low melting point agarose. Recovered DNA fragments are suitable for:
Ligation and transformation
Enzymatic restriction
Labelling by random primed or nick translation methods
Sequencing
产品优势：Quick and simple; extraction procedure requires approx. 45 min time and only a few hands-on steps.
Works with different agaroses and buffer systems; low melting point agarose is not required.
Efficient; specific binding of DNA allows easy removal of impurities.
Isolates large DNA fragments without shearing; silica particles are uniform in size and have smooth surfaces, ensuring recovery of intact DNA fragments （up to 100 kb）.
Even isolates oligonucleotides （≥20 bp）; narrow size distribution of the silica particles and absence of fines ensure high binding capacity of the matrix.
Free of fines and small particles; recovered product will not inhibit subsequent enzymatic reactions.
Good yield; see "Product Description" for typical recoveries.
产品介绍：Sample size: 100 mg gel slice （for 1 standard reaction）
Size range of recovered DNA:20 bp - 100 kbp
Typical recovery: 20 bp, 55%; 40 bp, 68%, 120 bp, 76%; 200 bp, 80%; 8 - 9 kb, 75%; larger than 10 kb, <60%
产品组成：Silica Matrix, for 100 standard reactions
Solubilization Buffer, 60 ml
Nucleic Acid Binding Buffer, 100 ml
Wash Buffer, 20 ml
产品原理：Briefly, the extraction procedure involves the following:
DNA fragments are separated by electrophoresis until all interesting DNA fragments are resolved. A razor blade or scalpel is used to excise a minimal amount of agarose that contains each fragment.
The agarose is then solubilized and the solution is mixed with a silica suspension that contains a chaotropic salt. Under the buffer conditions used, DNA binds the silica matrix.
Since the binding process is specific for nucleic acids, the DNA remains bound while impurities are removed with simple washing steps.
The DNA is then eluted from the matrix in low salt buffer or water. Recovered DNA is ready-to-use for downstream applications
