The pYES2-DEST52 Gateway® destination vector is designed for rapid cloning with a Gateway® entry clone using lambda phage site-specific recombination and subsequent expression in Saccharomyces cerevisiae. As part of the YES™ Vector Collection, pYES2-DEST52 carries the promoter and enhancer sequences from the GAL1 gene for regulated expression in S.cerevisiae. pYES2-DEST52 features:
2µ origin of replication for high-copy maintenance
gene for auxotrophic selection on economical minimal medium
C-terminal V5 epitope and polyhistidine（6xHis） tags for efficient detection and purification
tR sites for efficient recombination with any attL-flanked Gateway® entry vector
