The pBAD-DEST49 Gateway® destination vector is designed for rapid cloning with a Gateway® entry clone using lambda phage site-specific recombination and subsequent high-level, tightly regulated expression in E. coli. The pBAD-DEST49 vector features:
The araBAD promoter for tightly regulated expression in E. coliR sites for efficient recombination with any attL-flanked Gateway® entry vector
thioredoxin fusion for improved protein yield and solubility
Enterokinase cleavage site for efficient cleavage of the N-terminal fusion with EKMax™
C-terminal V5 and 6xHis tags for efficient detection and purification of fusion proteins
