Human Proinsulin/Proinsulin ELISA Kit

产品类型：
ELISA kit
研究领域：
Signal Transduction
产品概述：
EK00179E is a ready-to-use microwell,strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Proinsulin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Proinsulin The ELISA analytical biochemical technique of the EK00179E kit is based on Proinsulin antibody-Proinsulin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Proinsulin antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Proinsulin Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
细胞定位：
Proinsulin is synthesized in the endoplasmic reticulum, where it is folded and its disulfide bonds are oxidized. It is then transported to the Golgi apparatus where it is packaged into secretory vesicles, and where it is processed by a series of proteases to form mature insulin. Mature insulin has 35 fewer amino acids; 4 are removed altogether, and the remaining 31 form the C-peptide. The C-peptide is abstracted from the center of the proinsulin sequence; the two other ends (the B chain and A chain) remain connected by disulfide bonds.
分析类型：
Sandwich
检测方法：
spectrophotometrical
反应性：
Human
检测范围："

0.625-40pmol/mL
试剂盒组分：
Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
基因名称：
Proinsulin
功能：
Proinsulin is the prohormone precursor to insulin made in the beta cells of the islets of Langerhans, specialized regions of the pancreas. In humans, proinsulin is encoded by the INS gene.
应用：
For research use only
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