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Thromboxane B2, TXB2|Chicken Thromboxane B2, TXB2 ELISA试剂盒

产品信息
  • Chicken Thromboxane B2, TXB2 ELISA Kit
    96 Tests
    Operating instructions

    FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

    Synonyms
    Thromboxane B2,TXB2

    Search name
    Chicken TXB2 ELISA Kit, Chicken Thromboxane B2 ELISA Kit, TXB2 ELISA Kit, Thromboxane B2 ELISA Kit

    Intended use
    This immunoassay kit allows for the in vitro quantitative determination of Chicken Thromboxane B2 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

    Test principle
    The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thromboxane B2, During the reaction, Thromboxane B2 in the sample or standard competes with a fixed amount of biotin-labeled Thromboxane B2 for sites on a pre-coated Monoclonal antibody specific to Thromboxane B2. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Thromboxane B2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    PDF Manual download

      "(本产品仅供体外科研用途,不可用于临床诊断!)

     

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