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PGmatrix™ 3D cell culture

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  • Simply incredible 3D cell culture 

    PGmatrix™ is a powerful and yet easy-to-use tool for in-vitro 3D cell culture with high-throughput screening and accurate in-vivo prediction for life science research and development. PGmatrix is formulated from peptide solution, and is offered pre-mixed with basic cell culture medium (DMEM, MEM, RPMI, L15), or in custom formulas.

    PGmatrix is incredibly easy to use, and allows you to get accurate results faster. Unlike traditional 3D cell culture systems, PGmatrix requires no complicated steps for preparation, no pre-chilled gel solutions and no toxic cross-linking reagents.
     

    No acidic or chill pre-gel solution Mimics cell microenvironment
    Cells are easily encapsulated Fast hydrogel formation (5-30 min)
    Purified Synthetic Peptide in neutral pH Works with standard methodologies
    Cell suspension at room temperature Easy for cell harvest after 3D culture

     

    A regular PGmatrix research kit includes 6mL 1% W/V PGmatrix nanofiber solution and 2mL PGworks solution. Most cells grow well in PGmatrix concentration from 0.2-0.5% W/V).

     PGmatrix descriptions and uses

    PGmatrix has been used by researchers to create many different types of cell lines. The list below should help you decide what is the best product to order:
     

    Product Cell Lines tested in PepGel in 3D
    PGmatrix MEM Breast cancer cell MCF-7
    PGmatrix L-15 Breast cancer cells
    MDA-MB-231 & MDA-MB-468
    PGmatrix DMEM Head and neck squamous cell
    carcinoma HN5,
    Ham’ F12 skin cell,
    HeLa cancer cell
    Liver cells
    Endothelial cells
    Islet cells
    Mouse Stem cells
    Pig stem cells
    human fibroblast cell lines,
    human glioblastoma stem like cells
    T-cells - Jurkat cells,
    CHO-S
    PGmatrix RPMI ZR-75
    Prostate cancer cell (LNCaP)
    Islet cells,
    Lymphoma cells
    PGmatrix DMEM* GFs hiPSC derived from human fibroblast
    MSCs
    PGmatrix Customized Human ESCs, iPSCs, MSCs

    Case studies

    PGmatrix has been used successfully in the creation of cell lines from cancer cells and stem cells. Click on the images below to read about recent success stories.
     

    ZR-75
    MCF-7


    Researchers have used PGmatrix in the creation of other cell lines, including MDA-MB-231 and MDA-MB-468 human breast cancer cells, Head and neck squamous cell carcinoma HN5, Rat embryonic stem cell (ESC), and Rat induced pluripotent stem cell (iPSC).
     

    In addition, recently PGmatrix has been successfully used in cell lines for stem cells (MSC), skin cells (Ham’s F12), prostate cancer cells (LNCaP), and HeLa cancer cells.

     

    Other relevant publications include:

    3D-printing enabled micro-assembly of a microfluidic electroporation system for 3D tissue engineering.
    Lab Chip, 2019, 19, 2362.

    3D cell culture stimulates the secretion of in vivo like exosomes.
    Scientific ReportS | (2019) 9:13012.

    hiPSC-qualified PGmatrix™ – case study.

    Liang J, Liu G, Wang J, Sun XS Controlled release of BSA-linked cisplatin through a PepGel self-assembling peptide nanofiber hydrogel scaffold. Amino Acids: The Forum for Amino Acid, Peptide and Protein Research, 29 May 2017.

    If any questions please contact Amy for
    13651715049 (wechat) or 355887203@qq.com

     
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