规格：10μl质粒/300μl菌液启动子：CMV质粒分类：腺病毒载体质粒大小：6.1 kb质粒标签：原核抗性：Amp筛选标记：克隆菌株：Stbl3备注：The vector pAAV-IRES-hrGFP contains a dicistronic expression cassette in which the Renilla reniformis green fluorescent protein (hrGFP) gene is expressed as a second open reading frame downstream of the encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES). The hrGFP gene serves as a useful marker for titering the virus and ascertaining the infection efficiency for the desired target cell type. Another unique feature of this vector is the FLAG sequence (3x) at the c-terminal of MCS. The foreign gene could be cloned in frame with the tag and the protein could be further purified or detected by anti-FLAG antibody.
