Sphingosine 1 Phosphate ELISA KIT|Human

Sphingosine 1 Phosphate ELISA KIT|Human

Catalog  EF006568

Packing 48T/96T

Reactivity Human

Detect Range 3.125-200ng/ml

Sphingosine 1 Phosphate ELISA KIT Sensitivity< 1.875ng/ml

Application Sphingosine 1 Phosphate ELISA KIT|Human  is for detecting the concentration of Human Sphingosine 1 Phosphate in serum, plasma, tissue homogenates and other biological fluids.

Storage condition 6 month at 4 Centigrade

 

Principle of Sphingosine 1 Phosphate ELISA KIT|Human

The microtiter plate provided in Sphingosine 1 Phosphate ELISA KIT|Human has been pre-coated with an Human Sphingosine 1 Phosphate antibody specific to Human Sphingosine 1 Phosphate. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human Sphingosine 1 Phosphateantibody preparation specific for Human Sphingosine 1 Phosphate then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Sphingosine 1 Phosphate, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human Sphingosine 1 Phosphatein the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

*Please noted that Sphingosine 1 Phosphate ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!