Human Glycoprotein hormones alpha chain, CGA ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!
PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Glycoprotein hormones alpha chain,Luteinizing hormone alpha chain(LSH-alpha),Follitropin alpha chain,Follicle-stimulating hormone
alpha chain(FSH-alpha),Chorionic gonadotrophin subunit alpha(CG-alpha),Thyrotropin alpha chain,Thyroid-stimulating hormone alpha
chain(TSH-alpha),Lutropin alpha chain,Anterior pituitary glycoprotein hormones common subunit alpha,Choriogonadotropin alpha chain,;
Search Name
Human Glycoprotein hormones alpha chain ELISA Kit, Human CGA ELISA Kit, Human FSHA ELISA Kit, Human LHA ELISA Kit, Human
TSHA ELISA Kit, Human LSH-alpha ELISA Kit, Human FSH-alpha ELISA Kit,
glycoprotein hormones alpha chain; CG-ALPHA; FSHA; GPHA1; GPHa; LHA; TSHA; FSH-alpha; LSH-alpha; TSH-alpha; anterior
pituitary glycoprotein hormones common subunit alpha; choriogonadotropin alpha chain; chorionic gonadotrophin subunit alpha; chorionic
gonadotropin, alpha polypeptide; follicle-stimulating hormone alpha chain; follicle-stimulating hormone alpha subunit; follitropin alpha
chain; luteinizing hormone alpha chain; lutropin alpha chain; thyroid-stimulating hormone alpha chain; thyrotropin alpha chain;
glycoprotein hormones, alpha polypeptide
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human glycoprotein hormones alpha chain,Glycoprotein
hormones alpha chain concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological
fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycoprotein hormones alpha chain. Standards or
samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for
Glycoprotein hormones alpha chain and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and
incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Glycoprotein hormones alpha chain,
biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by
the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The
concentration of Glycoprotein hormones alpha chain in the samples is then determined by comparing the O.D. of the samples to the
standard curve.
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