Product Name:Human Homeobox protein SIX3, SIX3 ELISA KIT
Packing:96T
Catalog No.:ELI-52984h
Gene Name:SIX3
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Homeobox protein SIX3(SIX3
Alternative Name:SIX3,Homeobox protein SIX3(SIX3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Homeobox protein SIX3, SIX3 ELISA KIT allows for the in vitro quantitative determination of Homeobox protein SIX3(SIX3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Homeobox protein SIX3, SIX3 ELISA KIT has been pre-coated with an Homeobox protein SIX3(SIX3 antibody specific to Homeobox protein SIX3(SIX3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Homeobox protein SIX3(SIX3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Homeobox protein SIX3(SIX3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Homeobox protein SIX3(SIX3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
