Product Name:Human Transducin- like enhancer protein 4, TLE4 ELISA KIT
Packing:96T
Catalog No.:ELI-52130h
Gene Name:TLE4
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Transducin-like enhancer protein 4(TLE4
Alternative Name:TLE4,Transducin-like enhancer protein 4(TLE4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Transducin- like enhancer protein 4, TLE4 ELISA KIT allows for the in vitro quantitative determination of Transducin-like enhancer protein 4(TLE4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Transducin- like enhancer protein 4, TLE4 ELISA KIT has been pre-coated with an Transducin-like enhancer protein 4(TLE4 antibody specific to Transducin-like enhancer protein 4(TLE4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Transducin-like enhancer protein 4(TLE4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Transducin-like enhancer protein 4(TLE4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Transducin-like enhancer protein 4(TLE4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
