Product Name:Human Monoacylglycerol lipase ABHD12, ABHD12 ELISA KIT
Packing:96T
Catalog No.:ELI-49893h
Gene Name:ABHD12
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Monoacylglycerol lipase ABHD12(ABHD12
Alternative Name:ABHD12,Monoacylglycerol lipase ABHD12(ABHD12
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Monoacylglycerol lipase ABHD12, ABHD12 ELISA KIT allows for the in vitro quantitative determination of Monoacylglycerol lipase ABHD12(ABHD12 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Monoacylglycerol lipase ABHD12, ABHD12 ELISA KIT has been pre-coated with an Monoacylglycerol lipase ABHD12(ABHD12 antibody specific to Monoacylglycerol lipase ABHD12(ABHD12 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Monoacylglycerol lipase ABHD12(ABHD12 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Monoacylglycerol lipase ABHD12(ABHD12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Monoacylglycerol lipase ABHD12(ABHD12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
