Product Name:Human Fructosamine- 3- kinase, FN3K ELISA KIT
Packing:96T
Catalog No.:ELI-47346h
Gene Name:FN3K
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Fructosamine-3-kinase(FN3K
Alternative Name:FN3K,Fructosamine-3-kinase(FN3K
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Fructosamine- 3- kinase, FN3K ELISA KIT allows for the in vitro quantitative determination of Fructosamine-3-kinase(FN3K concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Fructosamine- 3- kinase, FN3K ELISA KIT has been pre-coated with an Fructosamine-3-kinase(FN3K antibody specific to Fructosamine-3-kinase(FN3K .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Fructosamine-3-kinase(FN3K and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Fructosamine-3-kinase(FN3K, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Fructosamine-3-kinase(FN3K in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
