Product Name:Human Kyphoscoliosis peptidase, KY ELISA KIT
Packing:96T
Catalog No.:ELI-46534h
Gene Name:KY
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Kyphoscoliosis peptidase(KY
Alternative Name:KY,Kyphoscoliosis peptidase(KY
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Kyphoscoliosis peptidase, KY ELISA KIT allows for the in vitro quantitative determination of Kyphoscoliosis peptidase(KY concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Kyphoscoliosis peptidase, KY ELISA KIT has been pre-coated with an Kyphoscoliosis peptidase(KY antibody specific to Kyphoscoliosis peptidase(KY .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Kyphoscoliosis peptidase(KY and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Kyphoscoliosis peptidase(KY, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Kyphoscoliosis peptidase(KY in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
