Product Name:Human Late cornified envelope protein 3B, LCE3B ELISA KIT
Packing:96T
Catalog No.:ELI-46499h
Gene Name:LCE3B
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Late cornified envelope protein 3B(LCE3B
Alternative Name:LCE3B,Late cornified envelope protein 3B(LCE3B
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Late cornified envelope protein 3B, LCE3B ELISA KIT allows for the in vitro quantitative determination of Late cornified envelope protein 3B(LCE3B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Late cornified envelope protein 3B, LCE3B ELISA KIT has been pre-coated with an Late cornified envelope protein 3B(LCE3B antibody specific to Late cornified envelope protein 3B(LCE3B .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Late cornified envelope protein 3B(LCE3B and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Late cornified envelope protein 3B(LCE3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Late cornified envelope protein 3B(LCE3B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
