Product Name:Human Phosphoserine phosphatase, PSPH ELISA KIT
Packing:96T
Catalog No.:ELI-35829h
Gene Name:PSPH
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Phosphoserine phosphatase(PSPH
Alternative Name:PSPH,Phosphoserine phosphatase(PSPH
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Phosphoserine phosphatase, PSPH ELISA KIT allows for the in vitro quantitative determination of Phosphoserine phosphatase(PSPH concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Phosphoserine phosphatase, PSPH ELISA KIT has been pre-coated with an Phosphoserine phosphatase(PSPH antibody specific to Phosphoserine phosphatase(PSPH .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Phosphoserine phosphatase(PSPH and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Phosphoserine phosphatase(PSPH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Phosphoserine phosphatase(PSPH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
