Product Name:Human Short- wave- sensitive opsin 1, OPN1SW ELISA KIT
Packing:96T
Catalog No.:ELI-35345h
Gene Name:OPN1SW
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Short-wave-sensitive opsin 1(OPN1SW
Alternative Name:OPN1SW,Short-wave-sensitive opsin 1(OPN1SW
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Short- wave- sensitive opsin 1, OPN1SW ELISA KIT allows for the in vitro quantitative determination of Short-wave-sensitive opsin 1(OPN1SW concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Short- wave- sensitive opsin 1, OPN1SW ELISA KIT has been pre-coated with an Short-wave-sensitive opsin 1(OPN1SW antibody specific to Short-wave-sensitive opsin 1(OPN1SW .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Short-wave-sensitive opsin 1(OPN1SW and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Short-wave-sensitive opsin 1(OPN1SW, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Short-wave-sensitive opsin 1(OPN1SW in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
