Product Name:Human Polycomb protein EED, EED ELISA KIT
Packing:96T
Catalog No.:ELI-32193h
Gene Name:EED
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Polycomb protein EED(EED
Alternative Name:EED,Polycomb protein EED(EED
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Polycomb protein EED, EED ELISA KIT allows for the in vitro quantitative determination of Polycomb protein EED(EED concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Polycomb protein EED, EED ELISA KIT has been pre-coated with an Polycomb protein EED(EED antibody specific to Polycomb protein EED(EED .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Polycomb protein EED(EED and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Polycomb protein EED(EED, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Polycomb protein EED(EED in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
