Human endogenous retrovirus group V member 1, ERVV1 ELISA KIT

Human endogenous retrovirus group V member 1, ERVV1 ELISA KIT

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!

PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

ERVV-1,ENVV1; HERV-V1; HERV-V_19q13.41 provirus ancestral Env polyprotein 1; endogenous retrovirus group V member 1; endogenous retrovirus group V, member 2; envelope glycoprotein ENVV1; envelope protein ENVV1; endogenous retrovirus group V member 1

 

Search name

Human ENVV1 ELISA KIT ,Human ERVV-1 ELISA KIT ,Human HERV ELISA KIT,Human HERV-V1 ELISA KIT ,Human HERV-V_19q13.41 provirus ancestral Env polyprotein 1 ELISA KIT ,Human endogenous retrovirus group V member 1 ELISA KIT ,Human endogenous retrovirus group V member 2 ELISA KIT ,Human envelope glycoprotein ENVV1 ELISA KIT ,Human envelope protein ENVV1 ELISA KIT ,Human endogenous retrovirus group V member 1 ELISA KIT

 

Intended use

Human ENVV1 ELISA KIT allows for the in vitro quantitative determination of Human HERV-V_19q13.41 provirus ancestral Env polyprotein 1concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in Human ENVV1 ELISA KIT has been pre-coated with an antibody specific to HERV-V_19q13.41 provirus ancestral Env polyprotein 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forHERV-V_19q13.41 provirus ancestral Env polyprotein 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HERV-V_19q13.41 provirus ancestral Env polyprotein 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HERV-V_19q13.41 provirus ancestral Env polyprotein 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 0.31-20ng/ml

Human ENVV1 ELISA KIT PDF Manual download

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