Product Name:Human Centrosome- associated protein CEP250, CEP250 ELISA KIT
Packing:96T
Catalog No.:ELI-25572h
Gene Name:CEP250
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Centrosome-associated protein CEP250(CEP250
Alternative Name:CEP250,Centrosome-associated protein CEP250(CEP250
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Centrosome- associated protein CEP250, CEP250 ELISA KIT allows for the in vitro quantitative determination of Centrosome-associated protein CEP250(CEP250 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Centrosome- associated protein CEP250, CEP250 ELISA KIT has been pre-coated with an Centrosome-associated protein CEP250(CEP250 antibody specific to Centrosome-associated protein CEP250(CEP250 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Centrosome-associated protein CEP250(CEP250 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Centrosome-associated protein CEP250(CEP250, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Centrosome-associated protein CEP250(CEP250 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
