Product Name:Human Prostacyclin receptor, PTGIR ELISA KIT
Packing:96T
Catalog No.:ELI-21993h
Gene Name:PTGIR
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Prostacyclin receptor(PTGIR
Alternative Name:PTGIR,Prostacyclin receptor(PTGIR
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Prostacyclin receptor, PTGIR ELISA KIT allows for the in vitro quantitative determination of Prostacyclin receptor(PTGIR concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Prostacyclin receptor, PTGIR ELISA KIT has been pre-coated with an Prostacyclin receptor(PTGIR antibody specific to Prostacyclin receptor(PTGIR .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Prostacyclin receptor(PTGIR and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Prostacyclin receptor(PTGIR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Prostacyclin receptor(PTGIR in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
