Product Name:Human Lens epithelial cell protein LEP503, LENEP ELISA KIT
Packing:96T
Catalog No.:ELI-21033h
Gene Name:LENEP
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Lens epithelial cell protein LEP503(LENEP
Alternative Name:LENEP,Lens epithelial cell protein LEP503(LENEP
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Lens epithelial cell protein LEP503, LENEP ELISA KIT allows for the in vitro quantitative determination of Lens epithelial cell protein LEP503(LENEP concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Lens epithelial cell protein LEP503, LENEP ELISA KIT has been pre-coated with an Lens epithelial cell protein LEP503(LENEP antibody specific to Lens epithelial cell protein LEP503(LENEP .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Lens epithelial cell protein LEP503(LENEP and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Lens epithelial cell protein LEP503(LENEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Lens epithelial cell protein LEP503(LENEP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
