Product Name:Human Phenylalanine- 4- hydroxylase, PAH ELISA KIT
Packing:96T
Catalog No.:ELI-20976h
Gene Name:PAH
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Phenylalanine-4-hydroxylase(PAH
Alternative Name:PAH,Phenylalanine-4-hydroxylase(PAH
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Phenylalanine- 4- hydroxylase, PAH ELISA KIT allows for the in vitro quantitative determination of Phenylalanine-4-hydroxylase(PAH concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Phenylalanine- 4- hydroxylase, PAH ELISA KIT has been pre-coated with an Phenylalanine-4-hydroxylase(PAH antibody specific to Phenylalanine-4-hydroxylase(PAH .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Phenylalanine-4-hydroxylase(PAH and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Phenylalanine-4-hydroxylase(PAH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Phenylalanine-4-hydroxylase(PAH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
