Product Name:Human Ornithine aminotransferase, mitochondrial, OAT ELISA KIT
Packing:96T
Catalog No.:ELI-21392h
Gene Name:OAT
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Ornithine aminotransferase(mitochondrial(OAT
Alternative Name:OAT,Ornithine aminotransferase(mitochondrial(OAT
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Ornithine aminotransferase, mitochondrial, OAT ELISA KIT allows for the in vitro quantitative determination of Ornithine aminotransferase(mitochondrial(OAT concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Ornithine aminotransferase, mitochondrial, OAT ELISA KIT has been pre-coated with an Ornithine aminotransferase(mitochondrial(OAT antibody specific to Ornithine aminotransferase(mitochondrial(OAT .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Ornithine aminotransferase(mitochondrial(OAT and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ornithine aminotransferase(mitochondrial(OAT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Ornithine aminotransferase(mitochondrial(OAT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
