Product Name:Human Regulated endocrine- specific protein 18, RESP18 ELISA KIT
Packing:96T
Catalog No.:ELI-18112h
Gene Name:RESP18
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Regulated endocrine-specific protein 18(RESP18
Alternative Name:RESP18,Regulated endocrine-specific protein 18(RESP18
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Regulated endocrine- specific protein 18, RESP18 ELISA KIT allows for the in vitro quantitative determination of Regulated endocrine-specific protein 18(RESP18 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Regulated endocrine- specific protein 18, RESP18 ELISA KIT has been pre-coated with an Regulated endocrine-specific protein 18(RESP18 antibody specific to Regulated endocrine-specific protein 18(RESP18 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Regulated endocrine-specific protein 18(RESP18 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Regulated endocrine-specific protein 18(RESP18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Regulated endocrine-specific protein 18(RESP18 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
