Human Pyruvate carboxylase, mitochondrial, Pc ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
PC,PYC,pyruvate carboxylase,PCB; pyruvate carboxylase mitochondrial; pyruvic carboxylase
Search name
Human PC ELISA KIT ,Human PYC ELISA KIT ,Human pyruvate carboxylase ELISA KIT ,Human PCB ELISA KIT ,Human pyruvate carboxylase mitochondrial ELISA KIT ,Human pyruvic carboxylase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human pyruvate carboxylase, mitochondrial,PYC concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to PYC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PYC and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PYC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PYC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
