Product Name:Human Adherens junction- associated protein 1, AJAP1 ELISA KIT
Packing:96T
Catalog No.:ELI-11973h
Gene Name:AJAP1
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Adherens junction-associated protein 1(AJAP1
Alternative Name:AJAP1,Adherens junction-associated protein 1(AJAP1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Adherens junction- associated protein 1, AJAP1 ELISA KIT allows for the in vitro quantitative determination of Adherens junction-associated protein 1(AJAP1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Adherens junction- associated protein 1, AJAP1 ELISA KIT has been pre-coated with an Adherens junction-associated protein 1(AJAP1 antibody specific to Adherens junction-associated protein 1(AJAP1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Adherens junction-associated protein 1(AJAP1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Adherens junction-associated protein 1(AJAP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Adherens junction-associated protein 1(AJAP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
