Product Name:Human Sperm acrosome- associated protein 5, SPACA5 ELISA KIT
Packing:96T
Catalog No.:ELI-12737h
Gene Name:SPACA5
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Sperm acrosome-associated protein 5(SPACA5
Alternative Name:SPACA5,Sperm acrosome-associated protein 5(SPACA5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Sperm acrosome- associated protein 5, SPACA5 ELISA KIT allows for the in vitro quantitative determination of Sperm acrosome-associated protein 5(SPACA5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Sperm acrosome- associated protein 5, SPACA5 ELISA KIT has been pre-coated with an Sperm acrosome-associated protein 5(SPACA5 antibody specific to Sperm acrosome-associated protein 5(SPACA5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Sperm acrosome-associated protein 5(SPACA5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Sperm acrosome-associated protein 5(SPACA5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Sperm acrosome-associated protein 5(SPACA5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
