Product Name:Human Abhydrolase domain- containing protein 3, ABHD3 ELISA KIT
Packing:96T
Catalog No.:ELI-12142h
Gene Name:ABHD3
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Abhydrolase domain-containing protein 3(ABHD3
Alternative Name:ABHD3,Abhydrolase domain-containing protein 3(ABHD3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Abhydrolase domain- containing protein 3, ABHD3 ELISA KIT allows for the in vitro quantitative determination of Abhydrolase domain-containing protein 3(ABHD3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Abhydrolase domain- containing protein 3, ABHD3 ELISA KIT has been pre-coated with an Abhydrolase domain-containing protein 3(ABHD3 antibody specific to Abhydrolase domain-containing protein 3(ABHD3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Abhydrolase domain-containing protein 3(ABHD3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Abhydrolase domain-containing protein 3(ABHD3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Abhydrolase domain-containing protein 3(ABHD3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
