Product Name:Human Death domain- containing protein CRADD, CRADD ELISA KIT
Packing:96T
Catalog No.:ELI-10437h
Gene Name:CRADD
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Death domain-containing protein CRADD(CRADD
Alternative Name:CRADD,Death domain-containing protein CRADD(CRADD
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Death domain- containing protein CRADD, CRADD ELISA KIT allows for the in vitro quantitative determination of Death domain-containing protein CRADD(CRADD concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Death domain- containing protein CRADD, CRADD ELISA KIT has been pre-coated with an Death domain-containing protein CRADD(CRADD antibody specific to Death domain-containing protein CRADD(CRADD .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Death domain-containing protein CRADD(CRADD and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Death domain-containing protein CRADD(CRADD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Death domain-containing protein CRADD(CRADD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
