Product Name:Human Ghrelin O- acyltransferase, MBOAT4 ELISA KIT
Packing:96T
Catalog No.:ELI-07500h
Gene Name:MBOAT4
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Ghrelin O-acyltransferase(MBOAT4
Alternative Name:MBOAT4,Ghrelin O-acyltransferase(MBOAT4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Ghrelin O- acyltransferase, MBOAT4 ELISA KIT allows for the in vitro quantitative determination of Ghrelin O-acyltransferase(MBOAT4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Ghrelin O- acyltransferase, MBOAT4 ELISA KIT has been pre-coated with an Ghrelin O-acyltransferase(MBOAT4 antibody specific to Ghrelin O-acyltransferase(MBOAT4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Ghrelin O-acyltransferase(MBOAT4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ghrelin O-acyltransferase(MBOAT4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Ghrelin O-acyltransferase(MBOAT4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
