Product Name:Human Holliday junction recognition protein, HJURP ELISA KIT
Packing:96T
Catalog No.:ELI-06925h
Gene Name:HJURP
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Holliday junction recognition protein(HJURP
Alternative Name:HJURP,Holliday junction recognition protein(HJURP
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Holliday junction recognition protein, HJURP ELISA KIT allows for the in vitro quantitative determination of Holliday junction recognition protein(HJURP concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Holliday junction recognition protein, HJURP ELISA KIT has been pre-coated with an Holliday junction recognition protein(HJURP antibody specific to Holliday junction recognition protein(HJURP .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Holliday junction recognition protein(HJURP and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Holliday junction recognition protein(HJURP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Holliday junction recognition protein(HJURP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
