Product Name:Human Galactoside- binding soluble lectin 13, LGALS13 ELISA KIT
Packing:96T
Catalog No.:ELI-06727h
Gene Name:LGALS13
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Galactoside-binding soluble lectin 13(LGALS13
Alternative Name:LGALS13,Galactoside-binding soluble lectin 13(LGALS13
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Galactoside- binding soluble lectin 13, LGALS13 ELISA KIT allows for the in vitro quantitative determination of Galactoside-binding soluble lectin 13(LGALS13 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Galactoside- binding soluble lectin 13, LGALS13 ELISA KIT has been pre-coated with an Galactoside-binding soluble lectin 13(LGALS13 antibody specific to Galactoside-binding soluble lectin 13(LGALS13 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Galactoside-binding soluble lectin 13(LGALS13 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Galactoside-binding soluble lectin 13(LGALS13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Galactoside-binding soluble lectin 13(LGALS13 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
