Human Secretory phospholipase A2 receptor, PLA2R1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Secretory phospholipase A2 receptor,PLA2-R,PLA2R, CLEC13C ,M-type receptor,180 kDa secretory phospholipase A2 receptor,C-type lectin domain family 13 member C, PLA2R1,PLA2R; CLEC13C; PLA2-R; PLA2G1R; PLA2IR; 180 kDa secretory phospholipase A2 receptor; phospholipase A2 receptor 1 180kDa, secretory phospholipase A2 receptor 180 kDa
Search name
Human Secretory phospholipase A2 receptor ELISA KIT ,Human PLA2-R ELISA KIT ,Human PLA2R ELISA KIT ,Human M-type receptor ELISA KIT ,Human 180 kDa secretory phospholipase A2 receptor ELISA KIT ,Human C-type lectin domain family 13 member C ELISA KIT ,Human PLA2R1 ELISA KIT ,Human PLA2R ELISA KIT ,Human CLEC13C ELISA KIT ,Human PLA2-R ELISA KIT ,Human PLA2G1R ELISA KIT ,Human PLA2IR ELISA KIT ,Human 180 kDa secretory phospholipase A2 receptor ELISA KIT ,Human phospholipase A2 receptor 1 180kDa ELISA KIT ,Human secretory phospholipase A2 receptor 180 kDa ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human secretory phospholipase a2 receptor,PLA2R concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to PLA2R. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PLA2R and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PLA2R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PLA2R in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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