Product Name:Human Progesterone- induced- blocking factor 1, PIBF1 ELISA KIT
Packing:96T
Catalog No.:ELI-05136h
Gene Name:PIBF1
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Progesterone-induced-blocking factor 1(PIBF1
Alternative Name:PIBF1,Progesterone-induced-blocking factor 1(PIBF1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Progesterone- induced- blocking factor 1, PIBF1 ELISA KIT allows for the in vitro quantitative determination of Progesterone-induced-blocking factor 1(PIBF1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Progesterone- induced- blocking factor 1, PIBF1 ELISA KIT has been pre-coated with an Progesterone-induced-blocking factor 1(PIBF1 antibody specific to Progesterone-induced-blocking factor 1(PIBF1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Progesterone-induced-blocking factor 1(PIBF1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Progesterone-induced-blocking factor 1(PIBF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Progesterone-induced-blocking factor 1(PIBF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
