Product Name:Human Gastric inhibitory polypeptide, GIP ELISA KIT
Packing:96T
Catalog No.:ELI-02964h
Gene Name:GIP
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Gastric inhibitory polypeptide(GIP
Alternative Name:GIP,Gastric inhibitory polypeptide(GIP
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Gastric inhibitory polypeptide, GIP ELISA KIT allows for the in vitro quantitative determination of Gastric inhibitory polypeptide(GIP concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Gastric inhibitory polypeptide, GIP ELISA KIT has been pre-coated with an Gastric inhibitory polypeptide(GIP antibody specific to Gastric inhibitory polypeptide(GIP .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Gastric inhibitory polypeptide(GIP and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Gastric inhibitory polypeptide(GIP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Gastric inhibitory polypeptide(GIP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
