Product Name:Human Cholesteryl ester transfer protein, CETP ELISA KIT
Packing:96T
Catalog No.:ELI-02747h
Gene Name:CETP
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Cholesteryl ester transfer protein(CETP
Alternative Name:CETP,Cholesteryl ester transfer protein(CETP
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Cholesteryl ester transfer protein, CETP ELISA KIT allows for the in vitro quantitative determination of Cholesteryl ester transfer protein(CETP concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Cholesteryl ester transfer protein, CETP ELISA KIT has been pre-coated with an Cholesteryl ester transfer protein(CETP antibody specific to Cholesteryl ester transfer protein(CETP .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Cholesteryl ester transfer protein(CETP and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cholesteryl ester transfer protein(CETP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Cholesteryl ester transfer protein(CETP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
