Product Name:Human Hepatocellular Carcinoma Related Protein 1 ELISA Kit
Packing:96T
Catalog No.:ELA-E4231h
Gene Name:HCRP1
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Hepatocellular Carcinoma Related Protein 1
Alternative Name:HCRP1,Hepatocellular Carcinoma Related Protein 1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Hepatocellular Carcinoma Related Protein 1 ELISA Kit allows for the in vitro quantitative determination of Hepatocellular Carcinoma Related Protein 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Hepatocellular Carcinoma Related Protein 1 ELISA Kit has been pre-coated with an Hepatocellular Carcinoma Related Protein 1 antibody specific to Hepatocellular Carcinoma Related Protein 1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Hepatocellular Carcinoma Related Protein 1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Hepatocellular Carcinoma Related Protein 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Hepatocellular Carcinoma Related Protein 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
