Human neurofilament protein ELISA Kit

Human neurofilament protein ELISA Kit

Product Name:Human neurofilament protein ELISA Kit
Packing:96T

Catalog No.:ELA-E1326h

Gene Name:NF

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:neurofilament protein

Alternative Name:NF,neurofilament protein
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human neurofilament protein ELISA Kit allows for the in vitro quantitative determination of neurofilament protein concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human neurofilament protein ELISA Kit has been pre-coated with an neurofilament protein antibody specific to neurofilament protein .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for neurofilament protein and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain neurofilament protein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of neurofilament protein in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!